CERTIFICATE

IMPACT FACTOR 2021

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Rapid identification of non-candida albicans candida (ncac) species isolated from female patients clinically diagnosed with vulvovaginitis by using multiplex pcr

Author: 
Neamat A. Al-Baiyati, Saife D. Al-Ahmer and Noor I. Al-Baiyati
Subject Area: 
Life Sciences
Abstract: 

Background: The majority of women with Candida vaginitis suffer from uncomplicated vaginitis characterized by sporadic attacks of mild to moderate severity due to C. albicans, and these attacks occur in healthy adult women without any predisposing factors. In contrast, approximately 10% of women suffer from complicated Candida vaginitis, in which attacks either are more severe, occur on a recurrent basis, or are due to non-albicans Candida species. Although several conventional methods are routinely used for determintion; however, Candida vaginitis or vulvovaginitis a challenge for patients and gynecologists. Aims and objectives: Aims of the current study were to find out the prevalence of candidial vulvovaginitis attributed due to Non-Candida albicans Candida (NCAC) species such as Candida glabrata, Candida tropiclis and Candida parapsilosis, and to use a multiplex PCR technique as a rapid identification method for these Non-Candida albicans Candida species isolated from female patients clinically diagnosed with vulvovaginitis. Materials and Methods: In this study vaginal swabs from 160 female patients were used for culture, Api Candida system, VITEK 2 system and multiplex PCR analysis. Multiplex PCR was performed with species-specific primers targeted to the ITS region of rRNA gene of C. glabrata, C. tropicalis and C. parapsilosis. The result of the multiplex PCR was compared with conventional culture, Api Candida system and VITEK 2 system methods. The positive multiplex PCR product was identified by presence of ~ 423 bp, ~357 bp and ~300 bp amplicons of ITS region of rRNA gene for the C. glabrata, C. tropicalis and C. parapsilosis, reaspectively. Results: Conventional methods of candidial culture, Api Candida system and VITEK 2 system, showed that to sum up 100 out of 160 vaginal swabs were detected for Candida species; To sum up 16 out of 100 vaginal swabs were detected for C. glabrata, 10 out of 100 vaginal swabs were detected for C. tropicalis, 6 out of 100 vaginal swabs were detected for C. parapsilosis and 37 out of 100 vaginal swabs were detected for other Candida species. Five of the 60 samples that were negative by conventional methods were positive by multiplex PCR. Statistical analysis revealed that the PCR to have a sensitivity of 95.5 % in the detection of Non-Candida albicans in vulvovaginitis. Conclusion: This multiplex PCR method provides a sensitive, rapid, and reliable alternative to conventional methods to identify Non-Candida albicans Candida species isolated from female patients clinically diagnosed with vulvovaginitis.

PDF file: 

CALL FOR PAPERS

 

ONLINE PAYPAL PAYMENT

IJMCE RECOMMENDATION

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

EDITORIAL BOARD

CHUDE NKIRU PATRICIA
Nigeria
Dr. Swamy KRM
India
Dr. Abdul Hannan A.M.S
Saudi Arabia.
Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran