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Analysis of genetic diversity in lentil (lens culinaris) using cytological character and protein profiling

Author: 
G.N. Sheikh, Showkat Ahmad, Rajdeep Kudesia, MK Shrivastav
Subject Area: 
Life Sciences
Abstract: 

Cytological character and protein profile of five accessions (IPL-406, IPL-81, DPL-62, DPL-15, and Sehore 74-3) of lentil (Lens culinaris) were estimated through polyacrylamide gel electrophoresis and using cytological characters viz., mitotic index. The present protein profile revealed that experimental accession IPL-406(L-1) is very close to to the accession Sehore-74-3(L-5) and DPL-62(L-3) and DPL-15 (L-4) are more close at molecular level as compared to other accessions. The greatest similarity coefficient (36.3%) was observed between IPL-406 and Sehore-74-3, while lowest similarity (15%) was observed between IPL-406 and DPL-15.At protein profile level a total of 46 protein bands were obtained, 44 protein bands show polymorphism (95.6%) and rest 2 bands show monomorphism (4.3%). Present study has also revealed that the mitotic index was highest in IPL-81 accession (L-2) i.e., 26.71% meaning that the accession has high power of division. The accession IPL-81 show highest germination percentage in first 24th hour and this accession also has highest content of protein i.e., 29.5% than accessions estimated.

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