Anti-angiogenic activity of aqueous extract of h. integrifolia in ehrlich ascites carcinoma model

Angiogenesis plays an important role in tumor formation and proliferation. The development of anti-angiogenic agents to block new blood vessel growth will inhibit metastasis and induce apoptosis of the cancer cells. Many natural health products that inhibit angiogenesis also manifest other anticancer activities. The present article focuses on phyto constituents bark extract of H. intergefolia have a high degree of anti-angiogenic activity. The aqueous leaf extract inhibits the Ehrlich ascites tumor cell proliferation by in-vivo. The antiangionenic activity of H. intergefolia was confirmed by its inhibition of angiogenesis in in-vivo assays, peritoneal and chorioallantoic membrane assay. Reduction in the levels of the cytokine VEGF and microvessel density count in the peritoneum of mice treated with H. intergefolia indicated that the plant extract decreased VEGF production and the cytokine induced neovascularization. Our preliminary results suggest anti-angiogenic activity of H. intergefolia. Further evidence-based research and chemical optimization of these compounds could further enhance the effectiveness of these plant-based medicines in angiotherapy.