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Anti-bacterial activities of lawsoniainermis and camellia sinensis against some human pathogenic bacteria

Author: 
Anti-bacterial activities of lawsoniainermis and camellia sinensis against some human pathogenic bacteria
Subject Area: 
Health Sciences
Abstract: 

Background: Nowadays there are several bacteria show resistant to many antibiotics, therefore the discovery of new and more efficient antibacterial agents is essential. Objective: to evaluate the antibacterial activities of Lawsoniainermis (henna) and Camelliasinensis (green tea) against some pathogenic bacteria. Methodology: Fresh plants Lawsoniainermis and Camelliasinensis were collected with assistant by a plant taxonomist at the medicinal and aromatic plants research institute in the national center of research in Khartoum, Sudan. Then the plants were chapped into small slides and shade dried. By using Cup plates method; different concentrations of Ethanolic, Methanolic and boiling water extracts of plants were examined against different micro-organisms including clinical isolates and laboratory standard bacterial strain of Staphylococcusaureus ATCC 25923, Escherichiacoli ATCC 25922, Proteusmirabilis ATCC 49565, Klebsiellapneumoniae ATCC 35657, Pseudomonasaeruginosa ATCC 27853. Clinical isolates of these strain obtained from patients attending the Omdurman teaching hospital, Sudan. Results: Lawsoniainermis show various antimicrobial activity against standard bacteria ranges from 9.9 mm to 22.9 mm, whilst against isolated organisms methanolic extract provide sensitivity zone ranging 4.6 mm to 12 mm for all isolates, whereby ethanolic and boiling water extract act only against S. aureusfrom 8 mm – 15 mm. Camelliasinensis show various antimicrobial activity against standard bacteria ranging 7.2 mm – 24 mm except against P.mirabilis ATCC 49565 the boiling water extract show no activity, whereas against isolated organisms provide activity ranging from 3.6 mm to 19.8 mm with exception again no activity against P.mirabilis. Also antibacterial activities of different antibiotics against both standard bacteria and isolated bacteria show various result from 0 to 38 mm. Conclusion: The present studyclarify the antibacterial activities of used plants. Therefore these results supply the use of theseplant as antibacterial agent.

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