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Anti-cancer potentials of mukia maderasapatana (linn) m. Roem leaf extracts against colo320 cells

Author: 
Mujeera Fathima, Aneesh Nair and Florida Tilton
Subject Area: 
Life Sciences
Abstract: 

Understanding traditional medicines better has been the priority for scientists looking for a safer alternative for their synthetic counterparts. In the present study, an attempt was made to understand the anti-cancer potentials of Mukia maderasaptana (Linn) M. Roem. leaf extracts against human colon cancer cell lines Colo320 DM. The plant materials were subjected to sequential cold percolation extraction in Hexane, Chloroform and Methanol solvents. The phytoconstituents were determined using standard procedures. MTT based tetrazolium microculture assay was performed to understand the cytotoxicity of the extracts against the colon cancer cell lines, which showed significant activity. Further, the apoptosis induction potential of the extracts in the cancer cells was determined by CASPASEs quantification assay which showed the upregulation of these enzymes vital to apoptosis. The experiments revealed apoptotic induction in the cells by the extracts.

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