
Staphylococcus aureus is one of the important bacterial pathogen causing a wide spectrum of infections. Eucalyptus species are well known as medicinal plants due to their biological and pharmacological properties. The aim of the present study was to determine the antibacterial activity of eucalyptus oil against clinical isolates of Staphylococcus aureus. The MIC of eucalyptus oil was appeared to be 0.06% for S. aureus. The eucalyptus oil is found to have antibacterial activity against S. aureus. However, its irritant properties has been evaluated before it is formulated for medicinal purpose.