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Antibiotic resistance profile of gram negative bacterial isolates collected from various hospital and labs of karachi, Pakistan

Author: 
Saima Mohsin and Asma Saeed
Subject Area: 
Life Sciences
Abstract: 

A total of 51 gram negative bacterial cultures including E.coli, Pseudomonas, Salmonella, Enterobacter and Klebsiella species were obtained from different labs and hospitals. The bacterial isolates were screened for their antibiotic resistance against six commonly used antibiotics i.e. Streptomycin, Sulzone, Cefotaxime, Fosfomycin, Amikacin, Tazocin at six different concentrations (3µg, 5µg, 10µg, 30µg, 50µg and 100µg/ml).The clinical isolates showed significant resistance to commonly used antibiotics and some of them were found to be multidrug resistant. The frequency of resistance of bacterial isolates to individual antibiotics is found to be 78% for Cefotaxime, 70% for Sulzone, 73% for Streptomycin, 52% for Fosfomycin, 48% for Tazocin and 23% for Amikacin. The resistance frequency of gram negative bacteria at a concentration of 100µg/ml of individual antibiotic was found to be 51% for Cefotaxime, 20% for Sulzone, 25% for Fosfomycin, 37% for Streptomycin, 4% for Tazocin and no resistance was noted in case of Amikacin.

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