The present investigation revealed that the volatile oil obtained from Tagetes patula contained 10 compounds and they were tested against the fourth instar larvae of Spodoptera litura for their antifeedant activity by leaf disc bioassay. Among the compounds tested Terpinolene was the most effective feeding deterrent agent against Spodoptera litura in the laboratory condition. The mean area fed 100 ppm / cm2 and 500 ppm/cm2. The all compounds tested the antifeedant activity in both concentration appreciable value were recorded.