Diacure a polyherbal formulation of eleven medicinal plants against some Microorganisms was investigated for this purpose methanol extract of diacure samples were in vitro with Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis, Micrococcus luteus, Escherichia coli and Candida albicans as test strains. The disc diffusion method was applied in the trial was found to be the most effective against all the test strains except M. luteus.