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Antioxidant and antibacterial activities of ethyl acetate extract from the thallus of Grateloupia doryphora collected from kovalum seashore, Tamilnadu

Author: 
Kanimozhi, S. and Sridhar, S.
Subject Area: 
Life Sciences
Abstract: 

The objective of this study was to determine the phytochemical content, antibacterial activity and antioxidant activity of ethyl acetate extract of Grateloupia doryphora. The ethyl acetate extract of G. doryphora was evaluated fortotal phenolic, flavonoid contents, antibacterial (agar disc diffusion and broth dilution methods) and antioxidant (ABTS assay, lipid peroxidation, superoxide radical scavenging, nitric oxide radical scavenging and reducing power) activities. The results indicated that ethyl acetate extract of G. doryphora was effective in inhibiting the growth of Gram positive viz; Staphylococcus aureus and Bacillus subtilis, gram negative viz; Pseudomonas aeruginosa and Klebsiella pneumoniae compared to antibiotic streptomycin. The ethyl acetate extract was found to be rich in total phenolic content, and high antioxidant activity as compared to Ascorbic acid. The presence of functional groups of active compounds was confirmed by Gas Chromatography mass spectroscopy (GSMS) analysis of ethyl acetate extract. It was concluded that all tested ethyl acetate extract of G. doryphora had antibacterial and antioxidant activities. These properties might be due to the presence of high total phenolic content or flavonoids. Hence, the ethyl acetate extract of thallus of G. doryphora represent a potential source of antibacterial and antioxidant compounds that may be used in food or pharmaceutical products.

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