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Assessing the efficacy and Cytotoxicity of four different root canal sealers

Author: 
Dr. Vanita Gautam and Dr. Hemant Kumar Halwai
Subject Area: 
Health Sciences
Abstract: 

Aim: to compare the efficacy and cytotoxicity of four different types of sealers including AH plus, Sankin, Tubliseal EWT and Apexit as well as their effect on cytokine release of L929 fibroblasts. Materials and Methods: Here cells were cultured in Complete Medium Culture (CMC) and then divided into two test groups. In group 1, sealers were added to cell culture wells immediately after mixing. In group 2, sealers were added to cell cultures 3 hours after mixing. Cell viability was evaluated by MTT assay after 4, 24 and 168 hours. The amount of Interleukin-6 (IL-6) released in response to the sealers was also evaluated by ELISA technique on fibroblasts after 24 hour period. Results: Significant differences were seen in cytotoxicity in both groups (P<0.001). The least cytotoxic sealers were AH Plus and Sankin respectively, whereas Tubliseal EWT showed the greatest cytotoxicity. The highest IL-6 level was observed in Tubliseal EWT and Sankin groups; which was statistically significant (P<0.001). Conclusion: AH plus has less cytotoxicity and induces less IL-6 release. Tubliseal EWT has greater cytotoxicity and induces more IL-6 release than other sealers.

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