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Assessment of genetic variation among different accessions of oat (avena sativa l.) using rapd markers

Author: 
Tasaduq H. Rather, Ashraf Khan, M., Maqsood A. Dar and Rajdeep Kudesia
Subject Area: 
Life Sciences
Abstract: 

The present study was carried out to assess the genetic variation in oats (Avena sativa L.) at DNA level. The three decamer primers, RAPD primers (GLD-18, GLE-08 and GLA-03) were used to estimate genetic diversity in Avena sativa genotypes. The assay revealed a large amount (65%) of polymorphism. The total of 20 bands were scored for three RAPD primers ranging from 4-9 corresponding to an average of 4.2 bands per primer and 13 bands of these were polymorphic. Polymorphic bands for each primer ranged from 0-100%. One primer out of three generated a total of 9 bands of which 9 (100%) were scored as polymorphic. Similarity matrix reveals the maximum similarity between varieties JHO 99-1 (S1) with JHO 822 (S3) and JHO 851 (S4) with JHO 2000-4 (S5) (Similarity Indices 0.880 and 0.903 respectively). While distantly related varieties were JHO 99-2 (S2) and JHO 99-1 (S1) (similarity indices 0.609). The RAPD cluster showed two major clusters namely cluster-I and cluster-II comprising of two varieties each. Cluster-I includes two cultivars namely JHO 99-1 (S1) and JHO 822 (S3). The major cluster-II includes JHO 851 (S4) and JHO 2000-4 (S5). The cultivar JHO 99-2 (S2) occupies a distinct place as revealed in the dendogram constructed. In present investigation RAPD markers revealed high degree of polymorphism (100%) among the five accessions of Avena varieties using GLE-08.

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