Jhansi which is situated in Central part of India of Bundelkhand region of Uttar Pradesh, India having two rivers namely Betwa and Pahuj in East and West part of the city respectively. The present investigation was carried out on both of the river to find out the assessment of water quality. The sampling locations were chosen carefully in order to get maximum representation of the diverse eco-hydrological environments within the river system. Our investigation showed that the water of Betwa and Pahuj River are alkaline in nature with higher concentration of cations. Nitrate was dominant at site III followed by Site II and I and it can be attributed due to high rate of decomposition and anthropogenic pressure. In both the river Dissolved Oxygen was dominant at site I and II compare to site III due to abundance of animated life and the microbial activity, which result in the depletion of the dissolved oxygen.