Saccharomyces cerevisiae was the cheapest strain available for the conversion of substrate. In the present study, it is used for bioethanol production from cashew apple juice. The influencing parameters that affect the production of bioethanol from cashew apple juice were optimized. Parameter optimization was done such as temperature, pH, inoculums size were studied, it was found to be pH of 4.5 temperature of 32°C and inoculum level 108 respectively. Under this optimum operating condition the maximum of yield 6.5 % bioethanol was achieved.