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Biological specifics of male gametophyte in feijoa sellowiana berg

Author: 
Kedelidze, N., Baratashvili, D., Meskhidze, A., Khalvashi, N. and Nakashidze, I.
Subject Area: 
Life Sciences
Abstract: 

The research deals with the biological specifics of the development of the male gametophyte in Feijoa sellowiana Berg. 1% agar-agar solution and solutions of sucrose of three different concentrations (20%, 30%, 40%) were used for the investigation. We germinated pollen gains in the Moist Chamber van Tieghem (hanging drop); to paint the pollen grains, the acetocarmine method was applied. Based on the results of the research the low fertility of the Feijoa pollen grains has been defined. 12 hours after having the seeds sowed in all cases only 1-2% of the pollen grains were germinated, whereas 34-47% was germinated after 24 hour period. In this case 40% sucrose solution appeared to be optimal. In case of sucrose solution of 20 and 30% the amount of the germinated pollen grains mounted 13-24% within 24 hours after being sowed. The shape and size of the pollen grains change less in accordance with being depended on the forms of Feijoa. In 98% cases the pollen grains have a triangular form. We also come across with roundish, elliptic and oval pollen grain forms. Their sizes range between 221- 262 mcm2.

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