Callus induction and shoot proliferation of jasmine (Jasminum sambac L. aiton.)

The present investigation was carried out on an important ornamental plant Jasminum sambac L. Aiton. belonging to family Oleaceae. The different vegetative parts i.e. nodal explants, shoot apices, stem and leaves were used to standardize the protocol through in vitro.The explants excised from field grown mature plant and thereafter planted on variously supplemented Murashige and Skoog’s medium for multiple shoot proliferation and callus induction. Early bud break (25-26 days) and maximum percentage of bud break (83.7) was observed in Jasminum sambac when MS medium supplemented with BAP (2.0 mg/l), Kn (1.0 mg/l), Ads (50 mg/l). Maximum percentage of multiple shoots was observed in Jasminum sambac on MS medium fortified with 2 mg/l BAP, 1.0 mg/l Kn and 50 mg/l Ads. Callus induction from leaf and meristem explants were achieved using different concentrations of auxins and cytokinins. Maximum percentage of cultures showing callus proliferation from leaf explants was observed in the media containing MS basal salts supplemented with 3.0mg/l BAP and 2.0 mg/l IAA in leaf explants. The percentage of cultures showing callus was similar in the media containing MS basal salts supplemented with 5.0 mg/l BAP and 2.0 mg/l NAA in leaf explants. However, white and friable callus were obtained in the media containing MS basal salts supplemented with 2.0mg/l BAP, 2.0 mg/l Kn and 2.0 mg/l IAA from meristem explants.