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Characterization of a bluetongue virus isolate from sheep of marathwada region in Maharashtra

Author: 
Audarya, S. D., Deshmukh, V. V., Gujar, M. B. and Bhonsle, A. V.
Subject Area: 
Health Sciences
Abstract: 

Bluetongue is an arthropod borne viral disease of ruminants. Bluetongue virus was isolated from clinically affected sheep of Marathwada region in Maharashtra. In initial studies inoculation of the isolate in chicken egg embryo by yolk sac route revealed characteristic cherry red discoloration and death of the chicken embryo. The isolate was adapted on BHK-21 cell line. The bluetongue virus isolate gave characteristic cytopathic effect (CPE) consisting of rounding of cells and cell detachment from 24 hours post inoculation. Further to confirm the bluetongue virus isolate by molecular characterization, the viral RNA was extracted and purified. The extracted RNA was subjected to electrophoresis in 1 % agarose gel. A total of 10 characteristic bands were observed upon electrophoresis thereby confirming the isolate. The results warrant continuous surveillance of sheep in Marathwada region of Maharashtra.

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