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Comparison of free lateral and deep margins of basal cell carcinoma (bcc) by the means of two staining methods: hematoxylin and eosin stain (h&e) and immunohistochemistry (ihc)

Author: 
Seirafi, H., Kamyab.Hesari Kambiz, Montazer, Fatemeh, Nikoo, Azita, Tavousi, Parvin, and Goodarzi, Azadeh
Subject Area: 
Health Sciences
Abstract: 

Background: BCC is one the common skin cancers. There are controversies regarding the best margin. The goal of this study was to determine margins with H&E and cytokeratin 17. Method: medical records of 96 patients reviewed. Pathologic specimens were reviewed and cytokeratin 17 was applied. Lateral and deep free margins were measured and compared by H&E and IHC in different subtypes of BCC. The extent and intensity of CK17 staining were examined too. Results: mean age of all patients was 66.3 years. 61 were male and 35 were female. Nose was the most common site of involvement. The most common type of the disease was infiltrative. Mean free lateral margin in H&E and IHC were significantly different in infiltrative and micronodular types (p<0.001 and 0.01, respectively). Mean free deep margin in H&E and IHC were significantly different in infiltrative, micronodular and nodular types (p =0.002, 0.04 and 0.04, respectively). The mean intensity and extent of CK 17 staining were not significantly different between various subtypes. (p=0.2 and 0.9, respectively) Conclusion: mean lateral and deep margins were not similar in two methods.

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