The recent challenges in computational biology are pertaining to Sequence Analysis and various tools related to it. Sequences are to be aligned before they are used for any other purpose like constructing the evolutionary or Phylogenetic trees. These trees predict the evolutionary relationship among various biological species or other entities based upon similarities and differences in their physical and/or genetic characteristics. The aligned sequences are helpful to know the unknown families from a known sequence structure and also for drug analysis. The present study aims at giving an insight about how to use the tools related to alignment and tree construction to study the evolutionary details of DNA and Protein sequences.