H. pylori is a microaerophilic bacterium that causes chronic gastritis in half the world's population. H. pylori is the etiological agent of 95% of duodenal ulcers and 70-80% of gastric ulcers and 60-70% of cases of gastric cancer. Routes have been proposed for the transmission of H. pylori infection are: fecal-oral, oral-oral, gastro-oral routes. Also it has been proposed water as a means for the transmission of H. pylori. There are few reports about whether H. pylori can be transmitted from animal reservoirs. Present study aimed to seek evidence the occurrence of H. pylori through detection of 16S rRNA gene of this bacterium in water where domestical turtles are kept.