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Detection of cryi ac gene expression in bt cotton through two dimensional agar gel precipitation test

Author: 
Shahzad Bashir, Shazia Anwer Bukhari, Sajjad ur Rehman, Sadia Javed and Waqass M, Bhutta
Subject Area: 
Life Sciences
Abstract: 

Hyper-immune serum was developed in three groups of rabbits. Bacillus thuringiensis (CEMB no. 30023 strain no. PR 17.2) was used to produce delta endotoxin, the product of Cry1 Ac gene which was used as antigen. Toxoid was prepared having 0 (control), 0.5 and 1.0 mg of delta endotoxin which was injected in rabbits which produced the hyper-immune serum. These antibodies were further used to detect the expression of CryI AC gene. Agarose gel precipitation test was done against standard antigen and antiserum, as well as against antiserum and delta endotoxin from the Bt plants. All the Agarose gel precipitation tests, showed positive results which indicated that antibodies were successfully developed and expressed in rabbits against delta endotoxin derived from B. thuringiensis.

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