Detection of newcastle disease virus (ndv) isolates from poultry by using reverse transcription polymerase chain reaction (rt-pcr)

Newcastle disease (ND) an infectious highly contagious avian disease causing severe economic losses to poultry worldwide and controlled by vaccination. In spite of regular vaccination a number of sporadic outbreaks are observed in vaccinated as well as non vaccinated birds and constant threat to poultry since last few years. Isolation and identification of circulating Newcastle disease virus isolates are crucial for the accurate diagnosis of the virus strains, which could be valuable for developing better prophylactic measures. This study was under taken to assess the prevalence of NDV in poultry farms. Among all the total of 39 collected field samples from various poultry farms the NDV was detected in seven field samples by RT-PCR. All the seven field samples yielded a band of 1662 bp on amplification of complete coding region of fusion protein gene of NDV. The results of this simple assay clearly indicated that the NDV strains are circulated in a range of susceptible birds.