There is a strong association between needlestick injuries and spread of infectious diseases. Dental health professionals are at constant risk of having these diseases. This research was designed to measure knowledge and awareness of needlestick injuries among dental health professionals in Riyadh. Data was collected from 777 participants who filled up a closed ended question format. Almost half of the participants reported one NSI during 12 months prior to the study, a few having experienced two or three and remaining with no injury experience. Insufficient clinical experience was reported to be the most common reason behind NSIs.