Considerable histological information on anther development was done by many scientists, but histochemical information on the anther development of Solanaceae members is almost lacking. An attempt is made to understand the distribution of histochemical substances during anther development in Solanum viarum. In the present study anther wall development is basic type, epidermis and endothecium is single layered and persistent. Middle wall layers are two in number, tapetum is binucleate glandular, dimorphic in nature, dual in origin. Rich content of RNA is seen in archesporial cells, sporogenous tissue, meiocyte, tapetal cells, wall layers and pollen grains and connective. It can be concluded that there is mutual interaction and utilization of biochemical substances in the anthers during formation and differentiation of pollen grains.