Totally six strains of Agrobacterium species was isolated from the soil samples of six different leguminous crop areas namely Kudikkadu, Thandangorai, Nadium, Athirampattinum, Buthalur, and Kattuthottam in Thanjavur Dist by using yeast extract mannitol agar (YEMA) medium. The isolated bacteria were characterized and identified as Agrobacterium rhizogenes based on the morphological, biochemical, cultural and pathogenicity tests. Biochemical characteristics such as total carbohydrate, protein, free amino acids, chlorophyll content and cholesterol were estimated from the agrobacterial isolates.