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Effect of genotype on shoot regeneration from hypocotyls, cotyledons and leaf explants from six cultivars of eggplant (solanum melongena l.)

Author: 
Jitendra Kumar Pal and Major Singh
Subject Area: 
Life Sciences
Abstract: 

Interaction of genotype and explants with medium, for their in vitro plant regeneration via shoot induction in eggplant (Solanum melongena) to select an elite cultivar with high shoot regeneration frequency for establish a protocol for commercial use in growing tissue culture industries, has been studied. Hypocotyls , cotyledon and leaf explants of six commercially grown Indian cultivars, Pant Rituraj, Punjab Sadabahar, IVBL-9, IVBR-3, IVBR-1, BRSPS-14, which vary in shape, size and color were used in this study. A combination of BA 0.5 mg L-1 and Kinetin 0.5 was found to be optimum for shoot regeneration from hypocotyl and cotyledon explants while BA with IBA was found to be optimum for leaf explants. Genotype, explant and genotype- explant interaction had highly significant effects on shoot regeneration with genotype making use of maximum effect on shoot regeneration. IVBR-1 was found to be most responsive genotype for regeneration of shoots from hypocotyls and cotyledon among the genotype while Punjab Sadabahar was found to be most responsive for regeneration of shoots from leaf explants. Among the explants hypocotyls yielded the maximum number of adventitious shoots followed by cotyledon and leaf. Rooting of regenerated shoots was achieved in basal MS medium. Complete plantlets were transferred to soil after initial hardening for 2 weeks under 70-80% relative humidity.

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