The present investigation was undertaken in order to know the effect of pre-sowing seed invigoration treatments on seed quality and seed yield in cotton genotype LRA 5166. The experiment was conducted in experimental farm, Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University during summer and winter seasons of the year 2010-12. The different pre-sowing invigoration seed treatments showed differential response for all the seed quality attributes, growth and yield parameters. Among the treatments, seed treated with 500 ppm GA3 (T3) recorded significantly higher field emergence percentage (92.33%), lesser days to first flower (35.33) and maximum seed cotton yield (154.27) when compared to winter season. The season had significantly effect on all the seed quality parameters studied for cotton.