Enhancement of FPase and β-Glucosidase Production by Trichoderma harzianum CKP01 in Carbon and Nitrogen Sources

Wastes are produced virtually by all types of industries. The drift in the world today is to convert waste into useful products through application of microorganisms. The current study aimed at the production of FPase and β- glucosidase enzyme by the filamentous fungi, Trichoderma harzianum CKP 01 in different carbon and nitrogen sources. A number of indigenously isolated fungi were screened for cellulolytic potential and Trichoderma harzianum CKP 01 was selected since, it showed maximum hydrolytic zone. Identifiation of the fungal isolate was done based on biochemical and molecular characterization by sequencing the 18S rRNA coding gene. Of the two enzymes FPase and β- glucosidase showed maximum activity and was in 2% dextrose (9.18 and 7.13 I Umg-1) at intracellular level and 2% sucrose (9.76 and 6.45 Umg-1) at extracellular level. Among different nitrogen sources, the highest activity was recorded in FPase (9.84 Umg-1) followed by β-glucosidase (7.72Umg-1) in 3.0% potassium nitrate when compared to control (0.71 I Umg-1) at an intracellular level. Potassium nitrate and peptone were the best nitrogen sources for the production of cellulase enzyme with FPase activity of 9.49Umg-1 and β-glucosidase of 6.62Umg-1 at an extracellular level by Trichoderma harzianum CKP 01 on 9th day of incubation.