The study of the extracellular enzymes activity, as protease, amylase and cellulase of bacteria isolated from the Kharaa River has been made. Proteases were also synthesized very intensively by bacterial strains isolated from the Kharaa River. Maximum amylase activity has been shown by the isolate from the sites with developed agriculture and livestock. The percentage of organisms having cellulytic activity is less in comparison with other enzymes; there are only few reports from bacterial strains. In the spring time, bacterial isolates have shown maximum cellulytic activity. Our study clearly revealed the potential of bacterial strains and their ability of extracellular enzyme production. Almost 92% of the strains have shown extracellular enzymatic activity for anyone of the enzyme screened.