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Estimation of cefpodoxime proxetil and ambroxol hydrochloride by first order derivative spectrophotometric method in pharmaceutical formulation

Author: 
Rote Ambadas Ranganath and Saudagar Ravindranath Bhanudas
Subject Area: 
Health Sciences
Abstract: 

A simple, precise, sensitive and accurate first order derivative spectrophotometric method was developed and validated for the simultaneous estimation of cefpodoxime proxetil and ambroxol hydrochloride in combined tablet dosage form. For determination ofsampling wavelengths, each of cefpodoxime proxetil and ambroxol hydrochloride were scanned in the wavelength range 200–400 nm in spectrum mode and sampling wavelengths were selected at 234.3 nm (zero crossing of cefpodoxime proxetil) where ambroxol hydrochloride showed considerable absorbance and at 248.2 nm (zero crossing of ambroxol hydrochloride) where cefpodoxime proxetil showed considerable absorbance. Beer’s law obeyed in the concentration range of 8-40 µg/ml for cefpodoxime proxetil and 5-25 µg/ml for ambroxol hydrochloride respectively. The correlation coefficients were found to be 0.995and 0.998 for cefpodoxime proxetil and for ambroxol hydrochloride respectively. Mean recoveries were found satisfactory.

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