Estimation of p-coumaric acid in aqueous extract of mimsoa pudica using HPLC-UV method

A detection technique was developed for the analysis of p-coumaric acid which is found to be in ester-linked form in Mimosa pudica (MP). Aqueous extract of MP was prepared by using Soxhlet apparatus and thereafter quantification was performed by HPLC with a UV-VIS detector. A reversed-phase C18 column was used as stationary phase and the optimal condition was established with (Water (50µl of formic acid pH adjusted to 3): acetonitrile (90:10)). To reduce the time of sample processing during quantification the extraction of ester-linked phenolic acid was performed using Soxhlet extraction technique our technique lead to rapid detection of p-coumaric acid using HPLC-UV. On quantification leaves of Mimosa pudica were found to contain 3.35% w/w of p-coumaric acid.