The present investigation is concentrated on Protein, carbohydrates and mineral content of Spathoglossum marginatum, Padina tetrasmatica, Turbinaria conoides, Ulva fasciata and Enteromorpha flexuosa for future applications in dietary supplements or food industries. Three seaweed samples (i) S. marginatum (ii) P. tetrasmatica (iii) T. conoides were collected from Mandapam coast in Rameshwaram and the two seaweeds (iv) U. fasciata (v) E. flexuosa were collected from Kovalam coast of Chennai, Tamilnadu, India. Of the above seaweeds tested S. marginatum showed maximum content of protein and carbohydrate. T. conoides showed maximum content of calcium, potassium and sodium, while U. fasciata showed maximum content of magnesium and iron. P. tetrasmatica observed maximum potassium content than other seaweeds tested