Background:-Use of FIBRIN GLUE as an alternative modality to surgery for the treatment of fistula in ano. Objectives:-To evaluate the influence of FIBRIN GLUE on healing of the fistula in ano. Patient and Method:- This prospective interventional study on 50 patients was carried out in the Department of Surgery, Jenen private hospital with different types of fistula in ano by using FIBRIN GLUE as an alternative modality to surgery for the treatment of fistula in ano. Results:- Fifty patients were studied, 42 male (84%) and 8 female (16%) and male to female ratio was 5.25:1, the age ranged from 11 to 70 years, with a mean age of 35 years + 5 years, the majority being in the 4th decade of life constituting 20 patient (40%). Low type 32 patients (64%) and high type 18 patients (36%). Thirty four patients (68%) were cured and 16 patients (32) were failed. Conclusion:- Use of FIBRIN GLUE as an alternative modality to surgery for the treatment of fistula in ano is a viable and attractive alternative method.