The investigation was taken up to study Genetic Distance between two breeds of cattle “Holstein and Jersey crossbred” by using Random amplified polymorphic DNA technique. This technique was carried out with 10 random primers. RAPD-PCR pattern revealed the total polymorphism loci of two breeds (67 loci), the total loci amplified in two breeds (118). The estimate of genetic distance was highest (2.21) with the primer (OP13) and the lowest (0.33) with the primer (OP15). The GD pooled over the primers was (1.40 ± 0.17) between these two breeds. The highest average percentage difference estimate (90.69) with the primer (OP15) and the lowest (19.81) with the primer (OP13), and the MAPD between two breeds estimate (54.41 ± 3.82).
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