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Green synthesis of silver nanoparticles using ocimum americanum aqueous leaf extract and study of its antimicrobial activity against esbl and mbl producing pathogens

Author: 
Meghana Gore, Mobashshera Tariq, Darshana Raut and Aruna, K.
Subject Area: 
Life Sciences
Abstract: 

The present study reports the synthesis of silver nanoparticles (Ag-NPs) using aqueous extract of Ocimum americanum (L.) leaf and silver nitrate (AgNO3) solution. The synthesized Ag-NPs were analysed using UV-Visible Spectroscopy (UV-Vis), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The UV-Vis spectrum ofAg-NPs in aqueous solution showed an absorbance peak around 424nm, which is a characteristic property of nanoparticle formation. The antimicrobial property of Ag-NPs was analyzed by qualitative methods like disc and well diffusion method against Extended Spectrum β-Lactamase (ESBL) and Metallo β-Lactamase (MBL) producing E.coli, Pseudomonas, Proteus, Citrobacter and Klebsiella species. The mean zones of inhibition for Ag-NPs and AgNO3were found to be in the range of 14-20mm for the test pathogens. Also the nanoparticle coated dressings showed antibacterial activity against the same, indicating its potential to control wound bio-burden and hence reduce the risk of further infection.

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