The present investigation entitled “A study on Health problems among female desktop computer users” was conducted with an aim to assess problems faced by female computer users in Ludhiana. The parameters included were physical characteristics of selected respondents and health problems encountered by them due to work stress. Ergonomic Stress Index worksheet was administered to the respondents in order to recognize the common pain symptoms experienced by the desktop computer users at workplace. Result revealed that majority of the respondents faced headache (61.25%), tiredness (61.25%) and 17 % of the respondents faced the problem of low motivation due work stress. As per Ergonomic Stress Index, the respondents felt moderate to mild pain in head, low back, neck, shoulder and dryness in eyes. So it can be concluded that prolonged hours of sitting in front computer leads to musculoskeletal problems.