The present study was conducted for period of 11 months to investigate the water quality and physico chemical parameters in the freshwater prawn Macrobrachium rosenbergii culture ponds, (Control and Experiment). The prawn groups of (mean length 12.8 ± 1.1 mm and mean weight 1.2 ± 0.2 mg) were fed with two different types of artificially prepared feed Viz, in control pond (Commercial prawn feed) and in experiment pond (Probiotic feed). Water temperature, pH, dissolved oxygen, NH3 and H2S were found better in trials with probiotics. Moreover, daily usage of probiotics in experiment pond was the best among trials. The best water quality was recorded in animal group fed with supplementary animal feed.