Isolation and identification of biofilm forming pseudomonas aeruginosa from wounds infection

Development of biofilm by Pseudomonas aeruginosa in wounds infection caused severe threat to patients and prevents the healing of wounds. To control such infection it’s important to characterize the pathogenic biofilm forming bacteria of wounds infection. The present study was aimed to isolate and identify the biofilm forming Pseudomonas aeruginosa from wounds infection. Ten P .aeruginosa were isolated from different wounds infection and identified based on morphological, biochemical and fatty acid methyl esterase analysis. Biofilm formation capability of isolates was studied by tube and microtiter plate methods. From the ten isolates the strain PA01, 02, 03 shown strong biofilm and strain PA04, 06, 10 moderate, while strain PA05, 07, 08, 09 showed weak biofilm formation on both methods except the strain PA10 shown strong biofilm on microtiter plate method.