Hibiscus rosa-sinensis leaves are rich in mucilage. But there are no reports on isolation and characterization of Hibiscus rosa-sinensis leaves mucilage. Hence, the present study was designed to isolate, purify and characterization of Hibiscus rosa-sinensis leaves mucilage. Hibiscus rosa-sinensis leaves mucilage was extracted, purified and identified by official methods. The isolated mucilage was characterized for physical, chemical and flow properties. The mucilage was further characterized by Fourier Transform Infrared Spectroscopy. Hibiscus rosa-sinensis leaves gave sufficient quantity of mucilage and it has good physical, chemical and flow properties. The Fourier Transform Infrared Spectroscopic study revealed that the mucilage has characteristic peaks. It was concluded that the Hibiscus rosa-sinensis leaves mucilage can be used as matrix forming material in pharmaceutical dosage forms.