Antimicrobial compound producing endophytic bacteria were isolated from a medicinal plant Vinca rosea. One of the isolated endophytes produced potential antimicrobial activity against some selected human pathogenic bacteria and a yeast. Morphological and biochemical characterizations indicated that the isolate, strain Vrb 46 was similar with Bacillus coagulans. TLC analysis of concentrated culture filtrate revealed several UV active bands on silica gel plate. Fermented cell free broth was extracted with chloroform-methanol and antimicrobial activity was found in the organic fraction.