Isolation of bacterial biosurfactants from local bacterial strains

Soil samples were collected from oil polluted station from Majnoon oil fieldat a latitude, 30°53'38.36"N and longitude of 47°32'26.18"E, Basrahcity, the samples were cultivated on Minimal salt medium contain crude oil as a sole source of carbon, four types of growing oil degraded bacteria identified according to their morphological and biochemical profiles as: Aeromonashydrophila, Bacillus subtilis, Pseudomonas aeruginosa and Pseudomonas fluorescens after using specific cultured media. The ability of defined species for production of biosurfactants have been checked by several methods, drops collapse test, blood haemolysis test and oil spreading test. All these bacterial specieswere determinate as biosurfactants producing species, the best results detected with Pseudomonas fluorescens, biosurfactants have been extracted and kept for further studies.