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Isolation, characterization & optimization of cultural condition for BACILLUS THURINGINSIS bt from soils of gaza strip

Author: 
Azmy Kh. Dagga, Mohamed H. Abdel Aziz, Abdelraouf A. Elmanama, Mervat Al-Sharif, Alaa Al jubb and Mahmoud W. El Hindi
Subject Area: 
Health Sciences
Abstract: 

Bacillus thuringiensis (Bt) is a ubiquitous Gram-positive, rod-shaped and sporulating bacterium that has been isolated worldwide from a great diversity of ecosystems including soil, water, dead insects, dust from silos. It makes proteins that are toxic to immature insects (larvae). In current study Bt was isolated from different agricultural and non-agricultural soils for different locations in Gaza strip. The effect of different parameters on biomass yield production such as pH, temperature and incubation time discuss. Present study show the optimum conditions of pH, temperature and incubation time, for growth as well as biomass production, and was obtained at pH 7.0, 30°C, 24 hours respectively. Morevore, compare between the Btg (isolated from Gaza soil) and Bti (refferance strain) the growth and yield of biomass production was similar.

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