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Marker-assisted breeding for resistance to bacterial leaf blight in popular fine grain variety Jagitial Sannalu

Author: 
Swathi, G., Durga Rani, CH.V., Jamal-Oddin, Arun Prem Kumar, N., Vanisri, S., Sheshu Madhav, M., Sundaram, R.M. Ranjith Kumar, N., Laha, M. S., Krishnam Raju S., Bhuvaneshwari, V. and Satyanarayana, P.V.
Subject Area: 
Life Sciences
Abstract: 

The present study focus on developing new Bacterial Blight resistant rice lines, using markers assisted backcrossing (MABC). A total of 536 SSR markers covering all / distributed throughout the 12 rice chromosomes were screened between the two parents for polymorphism, out of which 81 markers were found to polymorphic and all the 81 markers were screened in the breeding population. For each backcross generation of JGL1798/B95-1, the confirmed plants were screened with 81 polymorphic markers distributed on 12 chromosomes. From BC1F1 generation, two plants P28 and P44 with highest recipient alleles were chosen for the further backcrossing. From BC2F1 plants with the recipient alleles were selfed to produce BC2F2 and In BC2F2, fifty two plants were analyzed through phenotypic assays with different BLB cultures at two locations west Godavari and ARI Hyderabad. Five BC2F2 progenies possessing two BLB resistance genes in homozygous condition (xa13xa13Xa21Xa21) showed BLB resistance along with Jagitial Sannalu Characteristics. These five plants were chosen as the breeding lines for result of Bacterial Blight introgression.

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