Microbiological and biochemical analysis of different curd samples collected from different locations of Tamilnadu India were enumerated for their total bacteria. The higher bacterial population 3.1x105cfu M1-1 was found in Ruby and lower count 1.3x105 cfu M1-1 was recorded in Bhavani. The six different curd samples collected estimated for their fungi and all of them have no mold population. The total lactic acid bacteria in six different curd sample were enumerated. The higher total lactic acid bacterial population (1.8x105 cfu M1-1) in Ruby and lower count (1.0 x 105cfu ml-1) in Bhavani. The result of the nutritional value of curd estimating the protein and lactose were studied which indicated the highest value of protein and lactose in Bhavani sample notified was 18.20 g/500ml and 24.60 g/500ml respectively and least protein in Raja and Ruby sample notified was 17.60 g/500 ml and lactose content in Ruby sample notified was 23.40 g/500 ml respectively. The phosphatase test indicated that the microbial contamination was under limit in Bhavani and the pasteurization carried out during processing was good and microbial contamination was move that indicated the absence of phosphatase enzyme. As there are Good Manufacturing Practices, the curd produced by various dairies on commercial sale in generally good. From study Bhavani curd sample shows best result among the six samples.