Objective: to determine the difference in serum Molybdenum and Malondialdehyde levels among Myocardial Infarction (MI) patients and healthy control subjects. Methods: 60 MI patients and 60 healthy volunteers as control were included in this study. Serum levels of Molybdenum was determined using Electro thermal atomic absorption spectroscopy (ETAAS), whereas Malondialdehyde (MDA) level was measured using UV-VIS spectroscopy. Results: highly significant differences in serum Mo and MDA levels were observed between the (MI) patients and healthy control (P<0.0005) Conclusion: our study found that the highly increasing of Mo concentration in (MI) patients sera maybe used as a marker for Myocardial Infarction depending on its relationship with xanthine oxidase, which recently considered as a biomarker for Myocardial Infarction