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Optimization of submerged culture conditions for the enhanced production of Mycelial biomass of Pleurotus flabellatus

Author: 
Arul Kumar Murugesan, Karuna Sagaran Gunasagaran and Muthumary Johnpaul
Subject Area: 
Life Sciences
Abstract: 

The study investigates Pleurotus sp is potential growth in producing mycelium and enhancing biomass production for their stimulatory effects under submerged culture condition. Addition of 25.07 gL-1at the 21st day’s stage of exponential growth stationary phase give the most excellent stimulatory effect on growth of mycelium and fungal biomass production increase. The decline phase on 28th days under submerged culture biomass of dry weight production (20.50gL-1) level decrease. The optimized nutritional parameters were influencing the mycelia growth biomass productions enhance on carbon source in the form of glucose (23.71gL-1) at 21st days under submerged condition. Peptone (23.73gL-1) was found good nitrogen source for higher biomass production and stimulates the fungal growth. The effects of carbon/nitrogen (C/N) ratios were identified high yield biomass production on Glucose: Yeast Extract (G: YE) at 21st days (23.22gL-1). Phosphorus (P) form of Monosodium phosphate (NaH2PO4) and Potassium (K) in the form of Pottassium chloride(KCl) their involving process (P: 25.31gL-1; K: 22.79 gL-1) also change the growth and yield of biomass production between stationary phase and decline phase. The Magnesium (Mg) and Calcium (Ca) sources (Mg: 23.14 gL-1;Ca: 23.01gL-1) also specific changes and maximal mycelial growth as well as biomass production. The optimal media comparison of the environmental factors, Temperature at 30°C (26.22gL-1), pH 5.5 (23.22gL-1) and light at dark condition (24.34gL-1) under submerged culture for mycelial growth and production of biomass respectively. The potential use of mycelium growth and biomass production processes for can be achieve the future generations without human disorder disease from fungal metabolites.

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