The present study purports to assess the perceived sources of occupational stress among teachers with respect to gender The sample of the study consists of 100 teachers (males=64, females=36) belonging to various schools of District Aligarh. Occupational stress index by Srivastava & Singh was used in the present study. The data was analyzed by using t-test. The result revealed that male and female teachers significantly differs in Role Overload(RO), Role Ambiguity(RA), Unreasonable group & Political pressure(UG & PP), Under participation(UP) Poor peer relation(PPR), Impoverishment (IMP,) Unprofitability(UF)and Overall Occupational Stress whereas no significance difference was found between male and female teachers in Role Conflict(RC), Responsible for person(PR),Powerlessness (PL),Low Status( LS) and Strenuous Working condition(SWC)