Tridax procumbens Linn. is an important medicinal plant known as ‘akdandi’ used as an It is used as an antidiabetic, antibacterial, anti-inflammatory. The species is also well known in ayurvedic medicine for wound healing (R.Nia et al.).. The species is also well known in ayurvedic medicine for liver disorder. The present paper reveals the botanical standardization on the stem of T. procumbens. The pharmacognostic studies includes the phytochemistry, histochemistry, microscopic, macroscopic evaluation, percentage extractives, ash and acid insoluble ash, fluorescence analysis and estimation of polyphenol. The phytochemical and histochemical test includes starch, proteins, saponins, alkaloids, glycosides, sugar and flavonoid.