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Phenotypic and molecular characterization of SHV, tem, OXA and Extended-Spectrum β-lactamase produced by Klebsiella pneumonia isolates in a Adjara Hospital

Author: 
Koiava, T., Gonçalves, D., Palmeira, J., Arobelidze, K., Tediashvili, M., Akhvlediani, L. and Ferreira, H
Subject Area: 
Life Sciences
Abstract: 

The results marked the high prevalence of gene TEM.SHV, OXA, The majority of the strains carried two or more genes. This study points out the high resistance ESBL producing by Klebsiella pneumonia sample. A total of 28 clinical isolates of extended spectrum β- lactamase producing Klebsiellapneumoniae were sampled in Adjara region. The ESBL resistance was screened using disc diffusion method, while the resistance genes were detected by polymerase chain reaction (PCR). All sample were highly resistance to AML Amoxicilina, CTX Cefotaxima, FEP Cefepime, ATM Aztreonamo, CXM Cefuroxime, CAZ Ceftazidima, PRL Piperacilin, AUG Amox. + Ac. Clavulanico, TE Tetraciclin, STX Sulfamet. + Trimetrop-resistanec profile - 100%, F Nitrofurantoina, TOB Tobramicin- 84, 21%, ETP Ertapenemo- 78, 94%, C Cloranfenicol, CIP Ciprofloxacino, LEV Levofloxacin- 78, 94%, PIP/TAZ Piperacilin/Tazobactam, CN Gentamicina- 68, 42%, IMI Imipenemo, MRP Meropenemo, AK Amikacin - 57, 89%, NET Netilmicin-53%.

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