The aim of the present study was to investigate the Ethanol extract leaf from Jasminum sambac Linn. Qualitative analysis of showed the presence of alkaloids, carbohydrates, flavonoids, tannins, phenol, steroids, terpenoids and glycosides. Absence of leaves extracts protein, mucilage, saponins, fats and fixed oils. This work deals with the phytochemical screening and GC-MS, FT-IR studies of the Ethanol extract. The highest peak area of (40.87%) was obtained by 7-Tetradecenal, (Z) - (C14H26O) at retention time of (18.959) and the lowest peak area of (0.16%) was obtained by Phenol, 3, 5-bis (1,1-dimethylethyl)- (C14H22O) at retention time of (12.294). The FT-IR spectroscope studies shows different characteristic peak values of many functional groups in the extract. FT-IR analysis of leaf extract confirmed the presence of amide, alcohol, phenol, alkane, carboxylic acid, aldehyde, ketone, alkene, primary amine, aromatic esters, alkyl halide, and aliphatic amine compounds. This study summarizing the information about the phytochemical constituent’s presence in ethanolic leaf extracts and these constituents may be responsible for pharmacological activites.